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News

Agilent introduces new high sensitivity protein kit to speed sampling rates

Agilent Technologies Europe : 20 February, 2008  (New Product)
The Agilent High Sensitivity Protein 250 Kit has been introduced for the Agilent 2100 bioanalyzer to deliver more sensitivity than silver-stained SDS-polyacrylamide gel electrophoresis (SDS-PAGE).
The new instrument also provides quantitation capability along with the other advantages of a microfluidic lab-on-a-chip based kit.

The High Sensitivity Protein Kit, designed for both pharmaceutical QA/QC and life sciences research experiments, detects proteins down to as little 1pg/ul, comparable to or better than silver-staining slab gels. The kit features a dynamic range of four orders of magnitude for quantitation and covers a sizing range from 10 - 250kDa. For QA/QC applications, users can detect a 0.05 percent impurity while visualising the main target with reliable quantitation.

'This is the latest example of our programme to extend the sensitivity, speed, low sample consumption, ease of use and cost savings of the multi-purpose 2100 bioanalyzer system to additional life science applications,' said Knut Wintergerst, Agilent's Electrophoresis marketing manager. 'We think researchers will immediately recognise the advantages of replacing traditional silver-staining slab gels with a faster, more-reproducible and quantitative method.'

The new kit features a direct labelling reaction that is highly reproducible. Separation, quantitation and purity measurements can be performed in a single step, and typical throughput for QA/QC is 10 samples per hour.

Each High Sensitivity Protein 250 kit can be used to analyse 100 samples. The kit includes labelling dye and reagents, 10 Microfluidic chips (each capable of running 10 samples), bioanalyzer separation reagents, and user documentation. The Assay runs on the standard Agilent 2100 bioanalyzer system.
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