Free Newsletter
Register for our Free Newsletters
Newsletter
Zones
Analysis, Inspection and Laboratory
LeftNav
Assisted/Independent Living
LeftNav
Clinical and Nursing Equipment
LeftNav
Design and Manufacture of Medical Equipment
LeftNav
Diagnostics Equipment, Monitoring and Test
LeftNav
Education, Training and Professional Services
LeftNav
Health Education and Patient Management
LeftNav
Health Estates Management
LeftNav
Healthcare Support and Information Services
LeftNav
Hygiene and Infection Control
LeftNav
IT and Communications in Healthcare
LeftNav
Materials
LeftNav
Medical Device Technology
LeftNav
Research and Development
LeftNav
Safety and Security
LeftNav
View All
Other Carouselweb publications
Carousel Web
Defense File
New Materials
Pro Health Zone
Pro Health Zone
Pro Security Zone
Web Lec
Pro Engineering Zone
 
 
News

French researchers develop new way of observing DNA using AFM technology

Genopole : 10 December, 2007  (New Product)
Two French research teams have successfully developed a new method for observing DNA using atomic force microscopy (AFM) under intracellular physico-chemical conditions.
The teams were led by Eric Le Cam, from the Molecular Interactions and Cancer lab (UMR 8126 - Institut Gustave Roussy/CNRS/Universite Paris Sud) and David Pastre, from the Structure and Activity of Normal and Pathological Biomolecules - SABNP lab (Inserm/Universite d'Evry-Val-d'Essonne, U829, Genopole Evry).

The findings (Ref1 ) were validated by the observation of various degrees of DNA compaction - a key factor in replication and transcription. This method will enable research into DNA/protein interactions and their impacts on DNA activity, DNA chips and nanobiotechnology.

The first phase of these investigations was to find a method for absorbing DNA on a mica surface (a clay mineral traditionally used in biomolecular imaging), in the presence of monovalent salts. The findings, themselves representing a major advance in current techniques, make it possible to observe DNA molecules using atomic force Microscopy (AFM) under intracellular physico-chemical conditions (molecular crowding, monovalent salts). Until recently, it was impossible to obtain nanometric 3D high-resolution images of DNA molecules using AFM under such lifelike conditions.

To validate this method and demonstrate the relevance of these novel imaging conditions, researchers chose to examine how macromolecular crowding affected the compaction of DNA molecules. The presence of very large molecules in the intracellular environment impacts directly the degree of DNA compaction, and thus its activity. The phenomenon, which is well-known among biologists, but poorly studied has a critical influence on replication, transcription and, consequently, on gene expression.

The method enables the observation of DNA molecules in various physiological conditions, in particular in interaction with proteins; it can also be used in the fields of DNA chips or nanobiotechnology.

Ref 1: Atomic Force Microscopy Imaging of DNA under Macromolecular Crowding Conditions - David Pastre, Loic Hamon, Alain Mechulam, Isabelle Sorel, Sonia Baconnais, Patrick Curmi, Eric Le Cam, Olivier Pietrement, Biomacromolecules, 2007, Nov 19.
Bookmark and Share
 
Home I Editor's Blog I News by Zone I News by Date I News by Category I Special Reports I Directory I Events I Advertise I Submit Your News I About Us I Guides
 
   © 2012 ProHealthServiceZone.com
Netgains Logo