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News

LABORATORY AND ANALYTICAL – ANALYTICAL SOLUTIONS

Spark Holland : 29 May, 2009  (Special Report)
FOCUS ZONE REPORT Spark Holland develops a new method for low level determination of the 25-mono-hydroxy-metabolites of vitamin D2 and D3 in plasma.
The Assay is completely acetonitrile free avoiding problems associated with the current acetonitrile shortage.

Quantitation levels of around 1ng/mL are reported using the Symbiosis system for HPLC with integrated Solid Phase Extraction linked to an Applied Biosystems API 4000 Mass Spectrometer. The Assay involves almost no manual handling: plasma samples are diluted with a new protein disrupting buffer which also contains the internal standard. Samples are then placed in the autosampler for direct injection into the SPE-LC-MS/MS system. The method shows excellent linearity, precision and carry-over and runs in only four minutes, including SPE.

Low levels of vitamin D have been associated with osteoporosis, diabetes, hypertension and cancer. The new acetonitrile free vitamin D Assay is therefore likely to be of benefit, in terms of speed, cost effectiveness and convenience to clinical and clinical research laboratory environments.

An innovative development, the vitamin D Assay is the latest example of the high Assay quality and sensitivity achieved by dedicated on-line SPE-LC-MS/MS systems. High resolution SPE cartridges and precise SPE process control enable highly efficient sample preparation and concentration. Combined with direct elution into LC-MS/MS systems, high Assay performance is achieved as well as maximum Assay automation.
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