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News

NanoDrop 1000 spectrophotometer benefits HLA typing laboratories

Thermo Fisher Scientific - NanoDrop : 29 July, 2008  (New Product)
Thermo Fisher Scientific is to exhibit its new NanoDrop 1000 spectrophotometer that offers benefits for human leukocyte antigen (HLA) typing laboratories dealing with donor tissue matching.
The Thermo Scientific NanoDrop 1000 represents the growing trend of successful genome research tools finding their way to the clinical research setting. Specifically, the NanoDrop 1000 is utilised in the workflow as a replacement to time-consuming traditional spectrophotometers where efficiency and time-to-result are critical.

The NanoDrop 1000 is capable of carrying out full UV-Vis absorbance measurements with only 1ul of undiluted sample, enabling HLA laboratories to significantly speed up analysis time. The NanoDrop 1000 has been integrated into tissue typing workflows through a validation process as set by the HLA typing community. The NanoDrop 1000 spectrophotometer will be showcased for the first time at the Thermo Scientific booth 2739 at AACC 2008, being held in Washington DC, USA, from July 29 to 31, 2008.

The innovative cuvetteless method enables HLA laboratories to quickly and effectively process DNA samples without compromising yield. HLA is the major histocompatibility complex in humans, and laboratories performing HLA typing have distinct requirements for instrumentation, including preserving sample yield and optimizing analysis time.

The NanoDrop 1000 spectrophotometer has been integrated into the HLA typing workflow in a number of leading laboratories. DNA is extracted and purified from donor tissue and quantified on the NanoDrop 1000 using a patented sample retention system requiring only 1ul of sample. This quantitation method enables HLA laboratories to quickly and effectively process DNA samples without compromising yield.

In many HLA cases, donor workup results are required in less than four hours as the speed and accuracy of tissue analysis can affect the long-term Graft survival of the tissue. The NanoDrop 1000 spectrophotometer allows laboratories to analyse microsamples of DNA in only 10 seconds, reducing labour time and increasing laboratory productivity. Additionally, the novel microsample quantitation technology of the NanoDrop 1000 is critical for typing samples of limited cell mass, such as mononucleated bone marrow specimens.

In addition to reducing spectrophotometric quantitation prior to DNA amplification, the NanoDrop 1000 enables HLA typing laboratories to comply with the strict standards set by the ASHI. The NanoDrop 1000 has passed the rigorous validation process for HLA laboratories across the USA, and is now recommended by many certified laboratory inspectors.
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