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News

New structured illumination system offers fluorescence images with confocal quality

Olympus Life Science Europa - Microscopy : 04 March, 2008  (New Product)
Olympus has added the new advanced Optigrid M structured illumination system to the company’s life science microscopy range.
Optigrid M yields ultra-rich, multi-channel fluorescence and 3D/4D imaging, whilst still using standard illumination. Compatible with Olympus BX2, IX2 and MVX10 microscopes, Optigrid M controls are fully integrated into Olympus cell imaging software to provide all users with excellent confocal-like images.

Utilising the light emitted from an existing stabilised illumination source, the Optigrid M uses a one-dimensional optical grid mounted on a piezo-electronically driven actuator to project a line pattern onto the specimen. The grid is then moved perpendicularly to the grid lines, in 1/3 steps of its period length so that three grid movements result in one optical section.

The structured illumination process returns a strong signal wherever focus is sharp and a weak signal where focus is soft. Furthermore, a patented algorithm is used to combine these strong signals from the grid images so that each optical section contains data that is exactly within the focal plane.

A series of optical sections taken through a sample (z-stack) can then be combined to create a haze-free ultra-sharp composite image. Image stacks can also be used to produce 3D reconstructions using post-processing software.

The Optigrid M slider is inserted into the field diaphragm slot of a standard illuminator on upright (BX2), inverted (IX2) and Macroview MVX10 Olympus microscopes. The slider enables it to be used with any of the wavelengths available from the illumination system from UV to IR and also to be easily removed for standard Microscope functions.

The Optigrid M is fully controlled using Olympus cell software via a USB connection, which also ensures simple system integration. Furthermore, it provides users with excellent imaging throughput and programmable experiment repeatability for parallel multiple-specimen imaging.

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