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News

One-step RT-PCR kit delivers faster results on any real-time cycler

QIAGEN : 03 October, 2007  (New Product)
Qiagen’s QuantiFast Probe RT-PCR Kits have been developed to deliver faster results in real-time one-step RT-PCR with sequence-specific probes, such as TaqMan probes.
The kits are a new addition to the range of QuantiFast Kits, which provide fast cycling on any real-time cycler. With time savings of up to 60 percent on existing cyclers, there is no need for costly hardware upgrades.

A specially developed fast RT-PCR buffer containing the patent-pending additive Q-Bond greatly reduces denaturation, annealing, and extension times. The buffer also contains HotStarTaq Plus DNA Polymerase, which requires only five minutes at 95 degrees C for activation. The optimised QuantiFast RT Mix enables cDNA synthesis in just 10 minutes.

Accurate quantification of even low copy numbers is achieved with the kits. A balanced combination of KCl and NH4Cl in the fast RT-PCR buffer promotes specific primer annealing, enabling high PCR specificity and sensitivity. In addition, HotStarTaq Plus DNA Polymerase provides a stringent hot start, preventing the formation of nonspecific products.

QuantiFast Probe RT-PCR Kits are ready-to-use master mixes that eliminate the need for PCR optimisation. Simply follow the handbook protocol to get fast and reliable results. The kits are compatible with cyclers that use ROX dye for fluorescence normalisation (no optimisation of ROX concentration is necessary) and with cyclers that do not.
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