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News

Reagent formulation for efficient DNA transfer

Promega : 16 January, 2014  (New Product)
The Promega ViaFect trasfection reagent enables researchers to develop assays in more cell lines
Reagent formulation for efficient DNA transfer


Promega Corporation announces ViaFect Transfection Reagent, a tool for efficient DNA transfer into a wide variety of cell lines, including challenging suspension cells and stem cell-derived lines. ViaFect Transfection Reagent allows researchers to develop transfection-based assays in the cell line most relevant for their biology of interest.



Induced pluripotent stem cell (iPSC)-derived cell models, such as Cellular Dynamics International’s iCell Cardiomyocytes, show robust transfection with ViaFect Transfection Reagent, enabling assays not possible with other reagents.



This low toxicity reagent allows cells to stay healthy and metabolically active during transfection-based experiments, even at low cell densities, facilitating experiments where extended application of a treatment is needed. Cell viabilities of greater than 90% are routinely observed following transfection with ViaFect Transfection Reagent.



ViaFect Transfection Reagent requires minimal optimization with a simple three-step protocol. It does not require removal of serum, removal of culture medium or washing after introduction of the reagent/DNA complex. ViaFect Transfection Reagent contains no animal-derived components. For more information or to sample the new reagent, see: www.promega.com/ViaFect. A poster detailing results using ViaFect Transfection Reagent will also be presented at the Society for Laboratory Automation and Screening Conference and Exhibition to be held January 18th-22nd in San Diego, CA.


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